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KMID : 0359020080360010001
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Korean Journal of Gastrointestinal Endoscopy
2008 Volume.36 No. 1 p.1 ~ p.6
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Targeting Colon Cancer Cells with Fluorescent Magnetic Nanoparticles Conjugated to Anti-epidermal Growth Factor Receptor Antibodies
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Kim Chang-Wook
Cho Young-Seok
Yoon Tae-Jong
Kim Hyung-Keun
Kim Sung-Soo
Chang Ki-Yuk
Chae Hiun-Suk
Choi Myung-Gyu
Choi Gyu-Yong
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Abstract
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Background : The aim of this study was to evaluate targeting the epidermal growth factor receptors (EGFRs) of colon cancer cells with fluorescent magnetic nanoparticles (FMNP) conjugated to anti-EGFR antibodies.
Methods : The expression of EGFRs was evaluated in the HT-29 colon cancer cell lines and the control H-520 lung cancer cells by performing Western blot analysis. We synthesized silica-overcoated iron oxide nanoparticles that contained rhodamine B isothiocyanate (RITC) within a silica shell of a controllable thickness. This FMNP was conjugated to anti-EGFR mouse monoclonal antibody. The cells were treated with this probe conjugate for 4 hours and then the targeting was assessed via confocal microscopy. The fluorescence properties were evaluated for their binding to the expressed EGFRs with using a FACScan flow cytometer.
Results : EGFR was expressed in the HT-29 cells, as assessed by Western blot analysis. Red fluorescence was only detected in the membrane regions of the HT-29 cells on the confocal microscope imaging. On the FACS analysis, there was a significant shift of fluorescence intensity for the HT-29 cells.
Conclusion : Our data show the feasibility of targeting colon cancer cells with FMNP conjugated with anti-EGFR antibodies in vitro. (Korean J Gastrointest Endosc 2008;36:1-6)
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KEYWORD
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Colon cancer, Fluorescent magnetic nanoparticle (FMNP), EGFR
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